Transcript: Mouse NM_172674.2

Mus musculus PHD finger protein 20 (Phf20), mRNA.

Source:
NCBI, updated 2017-04-16
Taxon:
Mus musculus (mouse)
Gene:
Phf20 (228829)
Length:
5752
CDS:
184..3216

Additional Resources:

NCBI RefSeq record:
NM_172674.2
NBCI Gene record:
Phf20 (228829)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_172674.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000253535 CAGAAACCTCGCGCCTATTAT pLKO_005 2701 CDS 100% 15.000 21.000 N Phf20 n/a
2 TRCN0000253533 ATCGTGCCACTGATGATAAAC pLKO_005 3500 3UTR 100% 13.200 18.480 N Phf20 n/a
3 TRCN0000225874 TAAATTGCTGCACTATCATAT pLKO_005 1590 CDS 100% 13.200 18.480 N Phf20 n/a
4 TRCN0000218297 CAAAGGTGAGAGAGTACATTT pLKO_005 2885 CDS 100% 13.200 10.560 N Phf20 n/a
5 TRCN0000225875 CATCACGGTTCTGGATAATTT pLKO_005 4836 3UTR 100% 15.000 10.500 N Phf20 n/a
6 TRCN0000253536 GGAAGCTCGGGACCGTTTAAA pLKO_005 240 CDS 100% 15.000 10.500 N Phf20 n/a
7 TRCN0000265393 ATGACTCTGTGACGTTGAATG pLKO_005 1499 CDS 100% 10.800 7.560 N Phf20 n/a
8 TRCN0000225873 GTGGGTAATGCTAGGCCTAAA pLKO_005 610 CDS 100% 10.800 7.560 N Phf20 n/a
9 TRCN0000382197 GTGGGTAATGCTAGGCCTAAA pLKO_005 610 CDS 100% 10.800 7.560 N PHF20 n/a
10 TRCN0000253534 TCTGGTTCATACGGTTGATAC pLKO_005 1227 CDS 100% 10.800 7.560 N Phf20 n/a
11 TRCN0000245226 CAGTTCAACCTGCTCACACAT pLKO_005 2992 CDS 100% 4.950 3.465 N EG629235 n/a
12 TRCN0000245225 TCAGCACCAGTGGCAGTTCAA pLKO_005 2979 CDS 100% 4.950 3.465 N EG629235 n/a
13 TRCN0000218185 GAGTAGTTCAAACTGTCAAAC pLKO_005 551 CDS 100% 10.800 6.480 N Phf20 n/a
14 TRCN0000379949 AGCGTTGGAACCATCGTTATG pLKO_005 326 CDS 100% 10.800 15.120 N PHF20 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_172674.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_08258 pDONR223 100% 87.1% 88.5% None (many diffs) n/a
2 ccsbBroad304_08258 pLX_304 0% 87.1% 88.5% V5 (many diffs) n/a
3 TRCN0000481434 CCCTCCTTGTTAAAGCGGCATCAG pLX_317 14.2% 87.1% 88.5% V5 (many diffs) n/a
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