Construct: shRNA TRCN0000253537

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029074.3-220s21c1
DNA Barcode:
GTGCCTGGAACTGGCTAATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cnep1r1 (382030)
Gene Description:
CTD nuclear envelope phosphatase 1 regulatory subunit 1
Transcript:
RefSeq NM_029074.3 (CURRENT)
Match location:
Position 220 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000253537)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGCCTGGAACTGGCTAATAG
Hairpin Sequence:
5'-CCGG-GTGCCTGGAACTGGCTAATAG-CTCGAG-CTATTAGCCAGTTCCAGGCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGCCTGGAACTGGCTAATAGCTCGAGCTATTAGCCAGTTCCAGGCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGCCTGGAACTGGCTAATAGCTCGAGCTATTAGCCAGTTCCAGGCAC-3'

Other clones with same target sequence:

(none)