Construct: shRNA TRCN0000254240

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001127346.1-262s21c1
DNA Barcode:
CCGAGTGGGAAGCATGGATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ndufaf2 (75597)
Gene Description:
NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 2
Transcript:
RefSeq NM_001127346.1 (CURRENT)
Match location:
Position 262 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254240)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGTGGGAAGCATGGATTA
Hairpin Sequence:
5'-CCGG-CCGAGTGGGAAGCATGGATTA-CTCGAG-TAATCCATGCTTCCCACTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGTGGGAAGCATGGATTACTCGAGTAATCCATGCTTCCCACTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGTGGGAAGCATGGATTACTCGAGTAATCCATGCTTCCCACTCGG-3'

Other clones with same target sequence:

(none)