Construct: shRNA TRCN0000254408

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033485.2-1059s21c1
DNA Barcode:
TCGGTATCCTGCTTGAGTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
BC048644 (407789)
Gene Description:
cDNA sequence BC048644
Transcript:
RefSeq NM_001033485.2 (CURRENT)
Match location:
Position 1059 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254408)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGGTATCCTGCTTGAGTTTG
Hairpin Sequence:
5'-CCGG-TCGGTATCCTGCTTGAGTTTG-CTCGAG-CAAACTCAAGCAGGATACCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGGTATCCTGCTTGAGTTTGCTCGAGCAAACTCAAGCAGGATACCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGGTATCCTGCTTGAGTTTGCTCGAGCAAACTCAAGCAGGATACCGA-3'

Other clones with same target sequence:

(none)