Construct: shRNA TRCN0000254522

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026891.2-1784s21c1
DNA Barcode:
CAGCACCTCATGGATAGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdan1 (68968)
Gene Description:
congenital dyserythropoietic anemia, type I (human)
Transcript:
RefSeq NM_026891.2 (CURRENT)
Match location:
Position 1784 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254522)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCACCTCATGGATAGTTTA
Hairpin Sequence:
5'-CCGG-CAGCACCTCATGGATAGTTTA-CTCGAG-TAAACTATCCATGAGGTGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCACCTCATGGATAGTTTACTCGAGTAAACTATCCATGAGGTGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCACCTCATGGATAGTTTACTCGAGTAAACTATCCATGAGGTGCTG-3'

Other clones with same target sequence:

(none)