Construct: shRNA TRCN0000254566

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029942.1-198s21c1
DNA Barcode:
AGAATGTGGTTCCAGAGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prelid2 (77619)
Gene Description:
PRELI domain containing 2
Transcript:
RefSeq NM_029942.1 (CURRENT)
Match location:
Position 198 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254566)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAATGTGGTTCCAGAGATTT
Hairpin Sequence:
5'-CCGG-AGAATGTGGTTCCAGAGATTT-CTCGAG-AAATCTCTGGAACCACATTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAATGTGGTTCCAGAGATTTCTCGAGAAATCTCTGGAACCACATTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAATGTGGTTCCAGAGATTTCTCGAGAAATCTCTGGAACCACATTCT-3'

Other clones with same target sequence:

(none)