Construct: shRNA TRCN0000254673

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033493.2-2443s21c1
DNA Barcode:
TAGATACTAAAGACCATAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgrf2 (435529)
Gene Description:
adhesion G protein-coupled receptor F2
Transcript:
RefSeq NM_001033493.2 (CURRENT)
Match location:
Position 2443 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254673)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGATACTAAAGACCATAAAC
Hairpin Sequence:
5'-CCGG-TAGATACTAAAGACCATAAAC-CTCGAG-GTTTATGGTCTTTAGTATCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGATACTAAAGACCATAAACCTCGAGGTTTATGGTCTTTAGTATCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGATACTAAAGACCATAAACCTCGAGGTTTATGGTCTTTAGTATCTA-3'

Other clones with same target sequence:

(none)