Construct: shRNA TRCN0000254775

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013817.2-2153s21c1
DNA Barcode:
GATGATTACTCTTGGTCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sp140 (434484)
Gene Description:
Sp140 nuclear body protein
Transcript:
RefSeq NM_001013817.2 (CURRENT)
Match location:
Position 2153 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254775)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGATTACTCTTGGTCATAT
Hairpin Sequence:
5'-CCGG-GATGATTACTCTTGGTCATAT-CTCGAG-ATATGACCAAGAGTAATCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGATTACTCTTGGTCATATCTCGAGATATGACCAAGAGTAATCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGATTACTCTTGGTCATATCTCGAGATATGACCAAGAGTAATCATC-3'

Other clones with same target sequence:

(none)