Construct: shRNA TRCN0000254875

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001009952.2-296s21c1
DNA Barcode:
TACTATGGTCTTGACAATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Scgb2b20 (494519)
Gene Description:
secretoglobin, family 2B, member 20
Transcript:
RefSeq NM_001009952.2 (CURRENT)
Match location:
Position 296 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254875)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACTATGGTCTTGACAATATA
Hairpin Sequence:
5'-CCGG-TACTATGGTCTTGACAATATA-CTCGAG-TATATTGTCAAGACCATAGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACTATGGTCTTGACAATATACTCGAGTATATTGTCAAGACCATAGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACTATGGTCTTGACAATATACTCGAGTATATTGTCAAGACCATAGTA-3'

Other clones with same target sequence:

(none)