Construct: shRNA TRCN0000254930

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001029990.1-1432s21c1
DNA Barcode:
TGATCGCTCCATCCGAGTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mettl17 (52535)
Gene Description:
methyltransferase like 17
Transcript:
RefSeq NM_001029990.1 (CURRENT)
Match location:
Position 1432 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000254930)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATCGCTCCATCCGAGTTTC
Hairpin Sequence:
5'-CCGG-TGATCGCTCCATCCGAGTTTC-CTCGAG-GAAACTCGGATGGAGCGATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATCGCTCCATCCGAGTTTCCTCGAGGAAACTCGGATGGAGCGATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATCGCTCCATCCGAGTTTCCTCGAGGAAACTCGGATGGAGCGATCA-3'

Other clones with same target sequence:

(none)