Construct: shRNA TRCN0000255326

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001077421.1-244s21c1
DNA Barcode:
ATAACTGGACTGATGTCTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sbpl (638345)
Gene Description:
spermine binding protein-like
Transcript:
RefSeq NM_001077421.1 (CURRENT)
Match location:
Position 244 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255326)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATAACTGGACTGATGTCTATG
Hairpin Sequence:
5'-CCGG-ATAACTGGACTGATGTCTATG-CTCGAG-CATAGACATCAGTCCAGTTAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATAACTGGACTGATGTCTATGCTCGAGCATAGACATCAGTCCAGTTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATAACTGGACTGATGTCTATGCTCGAGCATAGACATCAGTCCAGTTAT-3'

Other clones with same target sequence:

(none)