Construct: shRNA TRCN0000255558

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024230.2-1477s21c1
DNA Barcode:
ACCTACATCCAGGAGCTATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smtnl1 (68678)
Gene Description:
smoothelin-like 1
Transcript:
RefSeq NM_024230.2 (CURRENT)
Match location:
Position 1477 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255558)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTACATCCAGGAGCTATAC
Hairpin Sequence:
5'-CCGG-ACCTACATCCAGGAGCTATAC-CTCGAG-GTATAGCTCCTGGATGTAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTACATCCAGGAGCTATACCTCGAGGTATAGCTCCTGGATGTAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTACATCCAGGAGCTATACCTCGAGGTATAGCTCCTGGATGTAGGT-3'

Other clones with same target sequence:

(none)