Construct: shRNA TRCN0000255765

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011629.3-605s21c1
DNA Barcode:
AGGATCCAAAGACTGCGTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nr2c1 (22025)
Gene Description:
nuclear receptor subfamily 2, group C, member 1
Transcript:
RefSeq NM_011629.3 (CURRENT)
Match location:
Position 605 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255765)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGATCCAAAGACTGCGTTAT
Hairpin Sequence:
5'-CCGG-AGGATCCAAAGACTGCGTTAT-CTCGAG-ATAACGCAGTCTTTGGATCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGATCCAAAGACTGCGTTATCTCGAGATAACGCAGTCTTTGGATCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGATCCAAAGACTGCGTTATCTCGAGATAACGCAGTCTTTGGATCCT-3'

Other clones with same target sequence:

(none)