Construct: shRNA TRCN0000255804

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001037804.1-205s21c1
DNA Barcode:
TATCCAACTCCGGTTCCCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DEFB130A (245940)
Gene Description:
defensin beta 130A
Transcript:
RefSeq NM_001037804.1 (CURRENT)
Match location:
Position 205 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000255804)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATCCAACTCCGGTTCCCAAA
Hairpin Sequence:
5'-CCGG-TATCCAACTCCGGTTCCCAAA-CTCGAG-TTTGGGAACCGGAGTTGGATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATCCAACTCCGGTTCCCAAACTCGAGTTTGGGAACCGGAGTTGGATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATCCAACTCCGGTTCCCAAACTCGAGTTTGGGAACCGGAGTTGGATA-3'

Other clones with same target sequence:

(none)