Construct: shRNA TRCN0000256521

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001040874.1-271s21c1
DNA Barcode:
ACAACAAGAAGACGCGCATCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H2AC19 (723790)
Gene Description:
H2A clustered histone 19
Transcript:
RefSeq NM_001040874.1 (CURRENT)
Match location:
Position 271 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256521)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAACAAGAAGACGCGCATCA
Hairpin Sequence:
5'-CCGG-ACAACAAGAAGACGCGCATCA-CTCGAG-TGATGCGCGTCTTCTTGTTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAACAAGAAGACGCGCATCACTCGAGTGATGCGCGTCTTCTTGTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAACAAGAAGACGCGCATCACTCGAGTGATGCGCGTCTTCTTGTTGT-3'

Other clones with same target sequence:

  1. TRCN0000097001