Transcript: Human NM_001040874.1

Homo sapiens H2A clustered histone 19 (H2AC19), mRNA.

Source:
NCBI, updated 2019-09-24
Taxon:
Homo sapiens (human)
Gene:
H2AC19 (723790)
Length:
534
CDS:
54..446

Additional Resources:

NCBI RefSeq record:
NM_001040874.1
NBCI Gene record:
H2AC19 (723790)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001040874.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000074588 CAACAAGAAGACGCGCATCAT pLKO.1 272 CDS 100% 4.950 2.475 Y H2AC18 n/a
2 TRCN0000242129 CAACAAGAAGACGCGCATCAT pLKO_005 272 CDS 100% 4.950 2.475 Y Hist1h2ad n/a
3 TRCN0000106848 CAACGACGAGGAACTGAACAA pLKO.1 320 CDS 100% 4.950 2.475 Y H2AC20 n/a
4 TRCN0000430088 CCGTACTGCTCCCTAAGAAGA pLKO_005 394 CDS 100% 4.950 2.475 Y H2AC18 n/a
5 TRCN0000256522 GAACAAGCTGCTGGGCAAAGT pLKO_005 335 CDS 100% 4.950 2.475 Y H2AC19 n/a
6 TRCN0000256523 TTGCTGCGCAAAGGCAACTAC pLKO_005 153 CDS 100% 4.950 2.475 Y H2AC19 n/a
7 TRCN0000097001 ACAACAAGAAGACGCGCATCA pLKO.1 271 CDS 100% 4.050 2.025 Y Hist2h2ac n/a
8 TRCN0000256521 ACAACAAGAAGACGCGCATCA pLKO_005 271 CDS 100% 4.050 2.025 Y H2AC19 n/a
9 TRCN0000257783 ACAAGAAGACGCGCATCATCC pLKO_005 274 CDS 100% 4.050 2.025 Y Hist1h2an n/a
10 TRCN0000429705 ACAAGAAGACGCGCATCATCC pLKO_005 274 CDS 100% 4.050 2.025 Y H2AC20 n/a
11 TRCN0000256519 ACGACGAGGAACTGAACAAGC pLKO_005 322 CDS 100% 4.050 2.025 Y H2AC19 n/a
12 TRCN0000418881 AGAGTCACCACAAGGCAAAGG pLKO_005 418 CDS 100% 4.050 2.025 Y H2AC18 n/a
13 TRCN0000074591 CTGAACAAGCTGCTGGGCAAA pLKO.1 333 CDS 100% 4.050 2.025 Y H2AC18 n/a
14 TRCN0000433959 CACAAGGCAAAGGGCAAGTGA pLKO_005 426 CDS 100% 3.000 1.500 Y H2AC18 n/a
15 TRCN0000257997 GACAACAAGAAGACGCGCATC pLKO_005 270 CDS 100% 2.250 1.125 Y Hist1h2af n/a
16 TRCN0000106847 CGAGGAACTGAACAAGCTGCT pLKO.1 326 CDS 100% 2.160 1.080 Y H2AC20 n/a
17 TRCN0000256520 TGCATCGCTTGCTGCGCAAAG pLKO_005 145 CDS 100% 2.000 1.000 Y H2AC19 n/a
18 TRCN0000074589 CGAGTATCTGACCGCCGAGAT pLKO.1 221 CDS 100% 1.350 0.675 Y H2AC18 n/a
19 TRCN0000106764 GCTGCTGGGCAAAGTCACCAT pLKO.1 341 CDS 100% 0.880 0.440 Y H2AC12 n/a
20 TRCN0000255257 CAAGAAGACGCGCATCATCCC pLKO_005 275 CDS 100% 0.720 0.360 Y Hist2h2ab n/a
21 TRCN0000417409 CATCATCCCTCGTCACCTCCA pLKO_005 287 CDS 100% 0.720 0.360 Y H2AC20 n/a
22 TRCN0000074592 GCCATCCGCAACGACGAGGAA pLKO.1 312 CDS 100% 0.000 0.000 Y H2AC18 n/a
23 TRCN0000097038 GCGCATCATCCCTCGTCACCT pLKO.1 284 CDS 100% 0.000 0.000 Y H2afj n/a
24 TRCN0000097031 GCTGGCCATCCGCAACGACGA pLKO.1 308 CDS 100% 0.000 0.000 Y Hist1h2ab n/a
25 TRCN0000074590 GCTGGCCTTCAGTTCCCGGTA pLKO.1 117 CDS 100% 0.000 0.000 Y H2AC18 n/a
26 TRCN0000106667 CTGGGCAAAGTCACCATCGCA pLKO.1 345 CDS 100% 0.250 0.125 Y H2AC13 n/a
27 TRCN0000097008 CCAAGGCCAAGTCGCGCTCTT pLKO.1 91 CDS 100% 0.000 0.000 Y H2afx n/a
28 TRCN0000335510 CCAAGGCCAAGTCGCGCTCTT pLKO_005 91 CDS 100% 0.000 0.000 Y H2afx n/a
29 TRCN0000106666 GCCGAGATCCTGGAGCTGGCT pLKO.1 234 CDS 100% 0.000 0.000 Y H2AC13 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001040874.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15630 pDONR223 0% 100% 100% None n/a
2 ccsbBroad304_15630 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000466536 CGATTGTCATTCGGCCGACTATTC pLX_317 51.9% 100% 100% V5 n/a
4 TRCN0000479809 GGTCGAATGGTCGCATTAGATTCC pLX_317 80.6% 99.2% 100% V5 9T>G;15C>T;21A>G n/a
5 ccsbBroadEn_15631 pDONR223 0% 98.9% 100% None (many diffs) n/a
6 ccsbBroad304_15631 pLX_304 0% 98.9% 100% V5 (many diffs) n/a
7 ccsbBroadEn_11266 pDONR223 100% 98.9% 100% None (many diffs) n/a
8 ccsbBroad304_11266 pLX_304 0% 98.9% 100% V5 (many diffs) n/a
9 TRCN0000473665 CTTGAACTGGACTCCCACCGCACC pLX_317 98.5% 98.9% 100% V5 (many diffs) n/a
10 ccsbBroadEn_01896 pDONR223 100% 94.3% 98.4% None (many diffs) n/a
11 ccsbBroad304_01896 pLX_304 0% 94.3% 98.4% V5 (many diffs) n/a
12 TRCN0000480994 TCCGAGTATGCGTATGATAAGCCC pLX_317 94.3% 94.3% 98.4% V5 (many diffs) n/a
13 ccsbBroadEn_01891 pDONR223 100% 87.4% 98.4% None (many diffs) n/a
14 ccsbBroad304_01891 pLX_304 0% 87.4% 98.4% V5 (many diffs) n/a
15 TRCN0000481190 CGTGTTGGCCGTTCGATTTAGAAT pLX_317 98.5% 87.4% 98.4% V5 (many diffs) n/a
16 ccsbBroadEn_04591 pDONR223 100% 86.6% 97.6% None (many diffs) n/a
17 ccsbBroad304_04591 pLX_304 0% 86.6% 97.6% V5 (many diffs) n/a
18 TRCN0000472223 CCTTGCTACGATGTATGAGACTTA pLX_317 100% 86.6% 97.6% V5 (many diffs) n/a
19 ccsbBroadEn_02055 pDONR223 100% 86.4% 98.4% None (many diffs) n/a
20 ccsbBroad304_02055 pLX_304 0% 86.4% 98.4% V5 (many diffs) n/a
21 TRCN0000466333 TGGGGTTTAATTGCATTCTTGTTA pLX_317 72.6% 86.4% 98.4% V5 (many diffs) n/a
22 ccsbBroadEn_15629 pDONR223 0% 86.4% 98.4% None (many diffs) n/a
23 ccsbBroad304_15629 pLX_304 0% 86.4% 98.4% V5 (many diffs) n/a
24 TRCN0000469353 AGACAGTAAACCCGGGATGCGCAC pLX_317 73.4% 86.4% 98.4% V5 (many diffs) n/a
25 ccsbBroadEn_01894 pDONR223 100% 86.1% 98.4% None (many diffs) n/a
26 ccsbBroad304_01894 pLX_304 0% 86.1% 98.4% V5 (many diffs) n/a
27 TRCN0000466135 ATCGATAACCATCGCTTACCTGGA pLX_317 72.6% 86.1% 98.4% V5 (many diffs) n/a
28 ccsbBroadEn_01895 pDONR223 100% 85.6% 98.4% None (many diffs) n/a
29 ccsbBroad304_01895 pLX_304 0% 85.6% 98.4% V5 (many diffs) n/a
30 TRCN0000468567 AACCATAGCTAACCCTGCACACAC pLX_317 100% 85.6% 98.4% V5 (many diffs) n/a
31 ccsbBroadEn_15628 pDONR223 0% 85.3% 96.1% None (many diffs) n/a
32 ccsbBroad304_15628 pLX_304 0% 85.3% 96.1% V5 (many diffs) n/a
33 TRCN0000474646 TCTACAGTCTCGTAGCTATGTTCA pLX_317 100% 85.3% 96.1% V5 (many diffs) n/a
34 ccsbBroadEn_07222 pDONR223 100% 85.3% 96.1% None (many diffs) n/a
35 ccsbBroad304_07222 pLX_304 0% 85.3% 96.1% V5 (many diffs) n/a
36 TRCN0000474296 CCGAGAACTTAGACACGTTCATCC pLX_317 98.5% 85.3% 96.1% V5 (many diffs) n/a
37 ccsbBroadEn_01893 pDONR223 100% 84.3% 98.4% None (many diffs) n/a
38 ccsbBroad304_01893 pLX_304 97.8% 84.3% 98.4% V5 (many diffs) n/a
39 TRCN0000469277 CTTGTACACGCATGGACCTTCTTT pLX_317 100% 84.3% 98.4% V5 (many diffs) n/a
40 ccsbBroadEn_09253 pDONR223 100% 84.1% 96.9% None (many diffs) n/a
41 ccsbBroad304_09253 pLX_304 0% 84.1% 96.9% V5 (many diffs) n/a
42 ccsbBroadEn_01892 pDONR223 100% 83.8% 98.4% None (many diffs) n/a
43 ccsbBroad304_01892 pLX_304 0% 83.8% 98.4% V5 (many diffs) n/a
44 TRCN0000473552 GACAAACTTCCATCCTCTTGAACC pLX_317 98.5% 83.8% 98.4% V5 (many diffs) n/a
45 ccsbBroadEn_03650 pDONR223 100% 82.8% 95.3% None (many diffs) n/a
46 ccsbBroad304_03650 pLX_304 0% 82.8% 95.3% V5 (many diffs) n/a
47 TRCN0000478286 ACAGCCCAAGTATGTTGCTGTGAT pLX_317 83.7% 82.8% 95.3% V5 (many diffs) n/a
48 ccsbBroadEn_00717 pDONR223 100% 75.8% 83.9% None (many diffs) n/a
49 ccsbBroad304_00717 pLX_304 0% 75.8% 83.9% V5 (many diffs) n/a
50 TRCN0000469289 TCCAAGCCCTCATTGGAGCTGGTC pLX_317 100% 75.8% 83.9% V5 (many diffs) n/a
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