Construct: shRNA TRCN0000256578

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001077637.1-3420s21c1
DNA Barcode:
TTTCCCTGATTACCAATATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB6D (150786)
Gene Description:
RAB6D, member RAS oncogene family
Transcript:
RefSeq NM_001077637.1 (CURRENT)
Match location:
Position 3420 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000256578)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTCCCTGATTACCAATATTA
Hairpin Sequence:
5'-CCGG-TTTCCCTGATTACCAATATTA-CTCGAG-TAATATTGGTAATCAGGGAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTCCCTGATTACCAATATTACTCGAGTAATATTGGTAATCAGGGAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTCCCTGATTACCAATATTACTCGAGTAATATTGGTAATCAGGGAAA-3'

Other clones with same target sequence:

(none)