Construct: shRNA TRCN0000257033

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021390.3-4891s21c1
DNA Barcode:
TTTGGCCTTGTAGGGTATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sall1 (58198)
Gene Description:
sal-like 1 (Drosophila)
Transcript:
RefSeq NM_021390.3 (CURRENT)
Match location:
Position 4891 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257033)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTGGCCTTGTAGGGTATATT
Hairpin Sequence:
5'-CCGG-TTTGGCCTTGTAGGGTATATT-CTCGAG-AATATACCCTACAAGGCCAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTGGCCTTGTAGGGTATATTCTCGAGAATATACCCTACAAGGCCAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTGGCCTTGTAGGGTATATTCTCGAGAATATACCCTACAAGGCCAAA-3'

Other clones with same target sequence:

(none)