Construct: shRNA TRCN0000257720
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_130870.1-339s21c1
- DNA Barcode:
- GGCTTTGGAGGCTATGGATAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Krtap19-2 (170651)
- Gene Description:
- keratin associated protein 19-2
- Transcript:
- RefSeq NM_130870.1 (CURRENT)
- Match location:
- Position 339 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000257720)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GGCTTTGGAGGCTATGGATAT
- Hairpin Sequence:
-
5'-CCGG-GGCTTTGGAGGCTATGGATAT-CTCGAG-ATATCCATAGCCTCCAAAGCC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGGCTTTGGAGGCTATGGATATCTCGAGATATCCATAGCCTCCAAAGCCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGGCTTTGGAGGCTATGGATATCTCGAGATATCCATAGCCTCCAAAGCC-3'
Other clones with same target sequence:
-
TRCN0000244012
