Construct: shRNA TRCN0000257793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026402.3-509s21c1
DNA Barcode:
CCATGCTACAAGCGGTGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atg3 (67841)
Gene Description:
autophagy related 3
Transcript:
RefSeq NM_026402.3 (CURRENT)
Match location:
Position 509 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGCTACAAGCGGTGTAAA
Hairpin Sequence:
5'-CCGG-CCATGCTACAAGCGGTGTAAA-CTCGAG-TTTACACCGCTTGTAGCATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGCTACAAGCGGTGTAAACTCGAGTTTACACCGCTTGTAGCATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGCTACAAGCGGTGTAAACTCGAGTTTACACCGCTTGTAGCATGG-3'

Other clones with same target sequence:

(none)