Construct: shRNA TRCN0000257847

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145134.3-925s21c1
DNA Barcode:
CTCAAGGGCAAGAAACTATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Spsb4 (211949)
Gene Description:
splA/ryanodine receptor domain and SOCS box containing 4
Transcript:
RefSeq NM_145134.3 (CURRENT)
Match location:
Position 925 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257847)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAAGGGCAAGAAACTATAC
Hairpin Sequence:
5'-CCGG-CTCAAGGGCAAGAAACTATAC-CTCGAG-GTATAGTTTCTTGCCCTTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAAGGGCAAGAAACTATACCTCGAGGTATAGTTTCTTGCCCTTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAAGGGCAAGAAACTATACCTCGAGGTATAGTTTCTTGCCCTTGAG-3'

Other clones with same target sequence:

(none)