Construct: shRNA TRCN0000257865

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178595.3-405s21c1
DNA Barcode:
TGGAGTCCGTTCCTGCATTAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ptrh1 (329384)
Gene Description:
peptidyl-tRNA hydrolase 1 homolog
Transcript:
RefSeq NM_178595.3 (CURRENT)
Match location:
Position 405 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257865)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGAGTCCGTTCCTGCATTAG
Hairpin Sequence:
5'-CCGG-TGGAGTCCGTTCCTGCATTAG-CTCGAG-CTAATGCAGGAACGGACTCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGAGTCCGTTCCTGCATTAGCTCGAGCTAATGCAGGAACGGACTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGAGTCCGTTCCTGCATTAGCTCGAGCTAATGCAGGAACGGACTCCA-3'

Other clones with same target sequence:

  1. TRCN0000234584