Construct: shRNA TRCN0000257913

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009431.2-3730s21c1
DNA Barcode:
ATGGGTTGGGAGGAGCTTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ctr9 (22083)
Gene Description:
CTR9 homolog, Paf1/RNA polymerase II complex component
Transcript:
RefSeq NM_009431.2 (CURRENT)
Match location:
Position 3730 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257913)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGGGTTGGGAGGAGCTTTAT
Hairpin Sequence:
5'-CCGG-ATGGGTTGGGAGGAGCTTTAT-CTCGAG-ATAAAGCTCCTCCCAACCCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGGGTTGGGAGGAGCTTTATCTCGAGATAAAGCTCCTCCCAACCCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGGGTTGGGAGGAGCTTTATCTCGAGATAAAGCTCCTCCCAACCCAT-3'

Other clones with same target sequence:

(none)