Construct: shRNA TRCN0000257962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177325.3-646s21c1
DNA Barcode:
GCCCGAAAGAAGCTAAGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tsr1 (104662)
Gene Description:
TSR1 20S rRNA accumulation
Transcript:
RefSeq NM_177325.3 (CURRENT)
Match location:
Position 646 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000257962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCGAAAGAAGCTAAGTAAA
Hairpin Sequence:
5'-CCGG-GCCCGAAAGAAGCTAAGTAAA-CTCGAG-TTTACTTAGCTTCTTTCGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCGAAAGAAGCTAAGTAAACTCGAGTTTACTTAGCTTCTTTCGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCGAAAGAAGCTAAGTAAACTCGAGTTTACTTAGCTTCTTTCGGGC-3'

Other clones with same target sequence:

  1. TRCN0000179212