Construct: shRNA TRCN0000262107

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024504.2-781s21c1
DNA Barcode:
TCAAGGACCTTTACCAGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dcun1d2 (102323)
Gene Description:
DCN1, defective in cullin neddylation 1, domain containing 2 (S. cerevisiae)
Transcript:
RefSeq NM_001024504.2 (CURRENT)
Match location:
Position 781 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262107)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAAGGACCTTTACCAGTTTA
Hairpin Sequence:
5'-CCGG-TCAAGGACCTTTACCAGTTTA-CTCGAG-TAAACTGGTAAAGGTCCTTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAAGGACCTTTACCAGTTTACTCGAGTAAACTGGTAAAGGTCCTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAAGGACCTTTACCAGTTTACTCGAGTAAACTGGTAAAGGTCCTTGA-3'

Other clones with same target sequence:

(none)