Construct: shRNA TRCN0000262726

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001082546.1-180s21c1
DNA Barcode:
ATTCGAAGCCGTTGAGTATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
BC100530 (100034684)
Gene Description:
cDNA sequence BC100530
Transcript:
RefSeq NM_001082546.1 (CURRENT)
Match location:
Position 180 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000262726)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTCGAAGCCGTTGAGTATAA
Hairpin Sequence:
5'-CCGG-ATTCGAAGCCGTTGAGTATAA-CTCGAG-TTATACTCAACGGCTTCGAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTCGAAGCCGTTGAGTATAACTCGAGTTATACTCAACGGCTTCGAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTCGAAGCCGTTGAGTATAACTCGAGTTATACTCAACGGCTTCGAAT-3'

Other clones with same target sequence:

(none)