Construct: shRNA TRCN0000263008

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001101320.1-191s21c1
DNA Barcode:
GTAGAAATGAGACGAACTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SERPINE3 (647174)
Gene Description:
serpin family E member 3
Transcript:
RefSeq NM_001101320.1 (CURRENT)
Match location:
Position 191 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263008)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGAAATGAGACGAACTTTG
Hairpin Sequence:
5'-CCGG-GTAGAAATGAGACGAACTTTG-CTCGAG-CAAAGTTCGTCTCATTTCTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGAAATGAGACGAACTTTGCTCGAGCAAAGTTCGTCTCATTTCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGAAATGAGACGAACTTTGCTCGAGCAAAGTTCGTCTCATTTCTAC-3'

Other clones with same target sequence:

(none)