Construct: shRNA TRCN0000263349

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020721.1-5931s21c1
DNA Barcode:
CTAGATAGATGCCGCAATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIAA1210 (57481)
Gene Description:
KIAA1210
Transcript:
RefSeq NM_020721.1 (CURRENT)
Match location:
Position 5931 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263349)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTAGATAGATGCCGCAATTAT
Hairpin Sequence:
5'-CCGG-CTAGATAGATGCCGCAATTAT-CTCGAG-ATAATTGCGGCATCTATCTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTAGATAGATGCCGCAATTATCTCGAGATAATTGCGGCATCTATCTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTAGATAGATGCCGCAATTATCTCGAGATAATTGCGGCATCTATCTAG-3'

Other clones with same target sequence:

(none)