Construct: shRNA TRCN0000263796

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001134875.1-747s21c1
DNA Barcode:
AGAGCCTTAGCCATCTGTCTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TEDC1 (283643)
Gene Description:
tubulin epsilon and delta complex 1
Transcript:
RefSeq NM_001134875.1 (CURRENT)
Match location:
Position 747 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000263796)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGCCTTAGCCATCTGTCTG
Hairpin Sequence:
5'-CCGG-AGAGCCTTAGCCATCTGTCTG-CTCGAG-CAGACAGATGGCTAAGGCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGCCTTAGCCATCTGTCTGCTCGAGCAGACAGATGGCTAAGGCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGCCTTAGCCATCTGTCTGCTCGAGCAGACAGATGGCTAAGGCTCT-3'

Other clones with same target sequence:

(none)