Construct: shRNA TRCN0000264282

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178399.4-884s21c1
DNA Barcode:
GCACAAGAAGAAGTCTGAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
3110035E14Rik (76982)
Gene Description:
RIKEN cDNA 3110035E14 gene
Transcript:
RefSeq NM_178399.4 (CURRENT)
Match location:
Position 884 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264282)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACAAGAAGAAGTCTGAATA
Hairpin Sequence:
5'-CCGG-GCACAAGAAGAAGTCTGAATA-CTCGAG-TATTCAGACTTCTTCTTGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACAAGAAGAAGTCTGAATACTCGAGTATTCAGACTTCTTCTTGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACAAGAAGAAGTCTGAATACTCGAGTATTCAGACTTCTTCTTGTGC-3'

Other clones with same target sequence:

  1. TRCN0000192679