Construct: shRNA TRCN0000264308

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028617.2-656s21c1
DNA Barcode:
ATCCAGTCTCTATGGCATATC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mvb12a (73711)
Gene Description:
multivesicular body subunit 12A
Transcript:
RefSeq NM_028617.2 (CURRENT)
Match location:
Position 656 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264308)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATCCAGTCTCTATGGCATATC
Hairpin Sequence:
5'-CCGG-ATCCAGTCTCTATGGCATATC-CTCGAG-GATATGCCATAGAGACTGGAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATCCAGTCTCTATGGCATATCCTCGAGGATATGCCATAGAGACTGGATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATCCAGTCTCTATGGCATATCCTCGAGGATATGCCATAGAGACTGGAT-3'

Other clones with same target sequence:

(none)