Construct: shRNA TRCN0000264352

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026177.3-1705s21c1
DNA Barcode:
TACGCCTTAAATATCCATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpalpp1 (67467)
Gene Description:
GPALPP motifs containing 1
Transcript:
RefSeq NM_026177.3 (CURRENT)
Match location:
Position 1705 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264352)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACGCCTTAAATATCCATAAT
Hairpin Sequence:
5'-CCGG-TACGCCTTAAATATCCATAAT-CTCGAG-ATTATGGATATTTAAGGCGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACGCCTTAAATATCCATAATCTCGAGATTATGGATATTTAAGGCGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACGCCTTAAATATCCATAATCTCGAGATTATGGATATTTAAGGCGTA-3'

Other clones with same target sequence:

(none)