Construct: shRNA TRCN0000264517

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019769.3-360s21c1
DNA Barcode:
AGAGGGAGAGGATCAGGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Chp1 (56398)
Gene Description:
calcineurin-like EF hand protein 1
Transcript:
RefSeq NM_019769.3 (CURRENT)
Match location:
Position 360 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264517)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGGGAGAGGATCAGGTAAA
Hairpin Sequence:
5'-CCGG-AGAGGGAGAGGATCAGGTAAA-CTCGAG-TTTACCTGATCCTCTCCCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGGGAGAGGATCAGGTAAACTCGAGTTTACCTGATCCTCTCCCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGGGAGAGGATCAGGTAAACTCGAGTTTACCTGATCCTCTCCCTCT-3'

Other clones with same target sequence:

(none)