Construct: shRNA TRCN0000264641

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177233.5-738s21c1
DNA Barcode:
TTGGAAGGAGAGGACTGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam19a4 (320701)
Gene Description:
family with sequence similarity 19, member A4
Transcript:
RefSeq NM_177233.5 (CURRENT)
Match location:
Position 738 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000264641)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGGAAGGAGAGGACTGTAAA
Hairpin Sequence:
5'-CCGG-TTGGAAGGAGAGGACTGTAAA-CTCGAG-TTTACAGTCCTCTCCTTCCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGGAAGGAGAGGACTGTAAACTCGAGTTTACAGTCCTCTCCTTCCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGGAAGGAGAGGACTGTAAACTCGAGTTTACAGTCCTCTCCTTCCAA-3'

Other clones with same target sequence:

(none)