Construct: shRNA TRCN0000265181

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028995.3-1314s21c1
DNA Barcode:
ATTCCGTTCGAGCCCTATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nipal3 (74552)
Gene Description:
NIPA-like domain containing 3
Transcript:
RefSeq NM_028995.3 (CURRENT)
Match location:
Position 1314 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265181)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTCCGTTCGAGCCCTATATT
Hairpin Sequence:
5'-CCGG-ATTCCGTTCGAGCCCTATATT-CTCGAG-AATATAGGGCTCGAACGGAAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTCCGTTCGAGCCCTATATTCTCGAGAATATAGGGCTCGAACGGAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTCCGTTCGAGCCCTATATTCTCGAGAATATAGGGCTCGAACGGAAT-3'

Other clones with same target sequence:

(none)