Transcript: Mouse NM_028995.3

Mus musculus NIPA-like domain containing 3 (Nipal3), transcript variant 2, mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Nipal3 (74552)
Length:
5025
CDS:
321..1553

Additional Resources:

NCBI RefSeq record:
NM_028995.3
NBCI Gene record:
Nipal3 (74552)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_028995.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000265181 ATTCCGTTCGAGCCCTATATT pLKO_005 1314 CDS 100% 15.000 21.000 N Nipal3 n/a
2 TRCN0000251420 CACTCTGTTTCCGGCTAATTA pLKO_005 2260 3UTR 100% 15.000 21.000 N Nipal3 n/a
3 TRCN0000251418 TCGGAGCACTCTTGGCCATTT pLKO_005 436 CDS 100% 10.800 15.120 N Nipal3 n/a
4 TRCN0000251419 GTGAAGCCTCCCAGATCTATG pLKO_005 1114 CDS 100% 10.800 7.560 N Nipal3 n/a
5 TRCN0000201551 CCTACTTCAAGACCAAGACAT pLKO.1 538 CDS 100% 4.950 3.465 N Nipal3 n/a
6 TRCN0000189696 GCTAGTGACATTTGCACCCAA pLKO.1 782 CDS 100% 2.640 1.848 N Nipal3 n/a
7 TRCN0000251421 GCGCCATCATCGGAATCATAT pLKO_005 670 CDS 100% 13.200 7.920 N Nipal3 n/a
8 TRCN0000202307 GAAGATGACAGGCGAGAACAT pLKO.1 812 CDS 100% 4.950 2.970 N Nipal3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_028995.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03805 pDONR223 100% 85.6% 89% None (many diffs) n/a
2 ccsbBroad304_03805 pLX_304 0% 85.6% 89% V5 (many diffs) n/a
3 TRCN0000491833 ATCAAACTGGTCTTATCCTTACAA pLX_317 32.3% 85.6% 89% V5 (many diffs) n/a
4 ccsbBroadEn_15943 pDONR223 0% 47.8% 46.8% None (many diffs) n/a
5 ccsbBroad304_15943 pLX_304 0% 47.8% 46.8% V5 (many diffs) n/a
6 TRCN0000467451 TCGGCCCATTGCACAGCGAACAAC pLX_317 59.1% 47.8% 46.8% V5 (many diffs) n/a
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