Construct: shRNA TRCN0000265532

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013318.3-4842s21c1
DNA Barcode:
CCCGTATTCCTCCTCGATTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRRC2B (84726)
Gene Description:
proline rich coiled-coil 2B
Transcript:
RefSeq NM_013318.3 (CURRENT)
Match location:
Position 4842 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265532)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGTATTCCTCCTCGATTTG
Hairpin Sequence:
5'-CCGG-CCCGTATTCCTCCTCGATTTG-CTCGAG-CAAATCGAGGAGGAATACGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGTATTCCTCCTCGATTTGCTCGAGCAAATCGAGGAGGAATACGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGTATTCCTCCTCGATTTGCTCGAGCAAATCGAGGAGGAATACGGG-3'

Other clones with same target sequence:

(none)