Construct: shRNA TRCN0000265850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001098418.1-221s21c1
DNA Barcode:
AGTGGAACCAGCAACACCTGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAGE12E (729431)
Gene Description:
G antigen 12E
Transcript:
RefSeq NM_001098418.1 (CURRENT)
Match location:
Position 221 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000265850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGGAACCAGCAACACCTGA
Hairpin Sequence:
5'-CCGG-AGTGGAACCAGCAACACCTGA-CTCGAG-TCAGGTGTTGCTGGTTCCACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGGAACCAGCAACACCTGACTCGAGTCAGGTGTTGCTGGTTCCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGGAACCAGCAACACCTGACTCGAGTCAGGTGTTGCTGGTTCCACT-3'

Other clones with same target sequence:

(none)