Transcript: Human NM_001098418.1

Homo sapiens G antigen 12E (GAGE12E), mRNA.

Source:
NCBI, updated 2019-05-01
Taxon:
Homo sapiens (human)
Gene:
GAGE12E (729431)
Length:
564
CDS:
117..470

Additional Resources:

NCBI RefSeq record:
NM_001098418.1
NBCI Gene record:
GAGE12E (729431)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001098418.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000369804 CTATGTACAGCCTCCTGAAAT pLKO_005 164 CDS 100% 13.200 6.600 Y GAGE4 n/a
2 TRCN0000255719 TACAGCCTCCTGAAATGATTG pLKO_005 169 CDS 100% 10.800 5.400 Y GAGE12F n/a
3 TRCN0000255892 TATGTACAGCCTCCTGAAATG pLKO_005 165 CDS 100% 10.800 5.400 Y GAGE12E n/a
4 TRCN0000197332 CCTGAAATGATTGGGCCTATG pLKO.1 177 CDS 100% 6.000 3.000 Y GAGE4 n/a
5 TRCN0000262200 ATGTACAGCCTCCTGAAATGA pLKO_005 166 CDS 100% 5.625 2.813 Y GAGE12D n/a
6 TRCN0000242733 CCTAGACCAAGGCGCTATGTA pLKO_005 150 CDS 100% 5.625 2.813 Y GAGE5 n/a
7 TRCN0000154274 GTACAGCCTCCTGAAATGATT pLKO.1 168 CDS 100% 5.625 2.813 Y GAGE4 n/a
8 TRCN0000161627 GTACAGCCTCCTGAAATGATT pLKO.1 168 CDS 100% 5.625 2.813 Y GAGE12I n/a
9 TRCN0000256137 TGTACAGCCTCCTGAAATGAT pLKO_005 167 CDS 100% 5.625 2.813 Y GAGE12H n/a
10 TRCN0000256038 ACTGGGTGTGAGTGTGAAGAT pLKO_005 366 CDS 100% 4.950 2.475 Y GAGE2D n/a
11 TRCN0000244667 CAGACTGGGTGTGAGTGTGAA pLKO_005 363 CDS 100% 4.950 2.475 Y GAGE4 n/a
12 TRCN0000137608 CCAAATCCAGAGGAGGTGAAA pLKO.1 414 CDS 100% 4.950 2.475 Y GAGE1 n/a
13 TRCN0000155993 CCAAATCCAGAGGAGGTGAAA pLKO.1 414 CDS 100% 4.950 2.475 Y GAGE2C n/a
14 TRCN0000115784 CCCGAGCAGTTCAGTGATGAA pLKO.1 201 CDS 100% 4.950 2.475 Y GAGE7 n/a
15 TRCN0000369735 CCGAGCAGTTCAGTGATGAAG pLKO_005 202 CDS 100% 4.950 2.475 Y GAGE4 n/a
16 TRCN0000138397 CGAGCAGTTCAGTGATGAAGT pLKO.1 203 CDS 100% 4.950 2.475 Y GAGE1 n/a
17 TRCN0000250095 CTAGACCAAGGCGCTATGTAC pLKO_005 151 CDS 100% 4.950 2.475 Y GAGE6 n/a
18 TRCN0000256139 CTGGGTGTGAGTGTGAAGATG pLKO_005 367 CDS 100% 4.950 2.475 Y GAGE12H n/a
19 TRCN0000137684 GAACCAGCAACACCTGAAGAA pLKO.1 225 CDS 100% 4.950 2.475 Y GAGE1 n/a
20 TRCN0000155031 GAACCAGCAACACCTGAAGAA pLKO.1 225 CDS 100% 4.950 2.475 Y GAGE2C n/a
21 TRCN0000115785 GCTATGTACAGCCTCCTGAAA pLKO.1 163 CDS 100% 4.950 2.475 Y GAGE7 n/a
22 TRCN0000369730 GGAACCAGCAACACCTGAAGA pLKO_005 224 CDS 100% 4.950 2.475 Y GAGE4 n/a
23 TRCN0000255891 GGGAACCAGCAACTCAATGTC pLKO_005 247 CDS 100% 4.950 2.475 Y GAGE12E n/a
24 TRCN0000255720 TAGACCAAGGCGCTATGTACA pLKO_005 152 CDS 100% 4.950 2.475 Y GAGE12F n/a
25 TRCN0000263104 ACAGCCTCCTGAAATGATTGG pLKO_005 170 CDS 100% 4.050 2.025 Y GAGE12C n/a
26 TRCN0000371197 ACCAGCAACACCTGAAGAAGG pLKO_005 227 CDS 100% 4.050 2.025 Y GAGE2D n/a
27 TRCN0000262203 AGACCAAGGCGCTATGTACAG pLKO_005 153 CDS 100% 4.050 2.025 Y GAGE12D n/a
28 TRCN0000255889 AGACTGGGTGTGAGTGTGAAG pLKO_005 364 CDS 100% 4.050 2.025 Y GAGE12E n/a
29 TRCN0000243152 AGTGTGAAGATGGTCCTGATG pLKO_005 376 CDS 100% 4.050 2.025 Y GAGE2E n/a
30 TRCN0000256037 AGTTCAGTGATGAAGTGGAAC pLKO_005 208 CDS 100% 4.050 2.025 Y GAGE2D n/a
31 TRCN0000370840 ATGAAGTGGAACCAGCAACAC pLKO_005 217 CDS 100% 4.050 2.025 Y GAGE6 n/a
32 TRCN0000262202 CAGCAACTCAATGTCAGGATC pLKO_005 253 CDS 100% 4.050 2.025 Y GAGE12D n/a
33 TRCN0000281541 CGCTATGTACAGCCTCCTGAA pLKO_005 162 CDS 100% 4.050 2.025 Y GAGE12G n/a
34 TRCN0000243156 CTGAAATGATTGGGCCTATGC pLKO_005 178 CDS 100% 4.050 2.025 Y GAGE2E n/a
35 TRCN0000256337 CTGAAGCTCATAGCCAGGAAC pLKO_005 331 CDS 100% 4.050 2.025 Y GAGE2A n/a
36 TRCN0000256136 GAACCAGCAACTCAATGTCAG pLKO_005 249 CDS 100% 4.050 2.025 Y GAGE12H n/a
37 TRCN0000256138 GAAGCTCATAGCCAGGAACAG pLKO_005 333 CDS 100% 4.050 2.025 Y GAGE12H n/a
38 TRCN0000265695 GAGCAGTTCAGTGATGAAGTG pLKO_005 204 CDS 100% 4.050 2.025 Y GAGE12F n/a
39 TRCN0000115783 GAGTGTGAAGATGGTCCTGAT pLKO.1 375 CDS 100% 4.050 2.025 Y GAGE7 n/a
40 TRCN0000137973 GAGTGTGAAGATGGTCCTGAT pLKO.1 375 CDS 100% 4.050 2.025 Y GAGE1 n/a
41 TRCN0000155220 GATGAAGTGGAACCAGCAACA pLKO.1 216 CDS 100% 4.050 2.025 Y GAGE2C n/a
42 TRCN0000162881 GATGAAGTGGAACCAGCAACA pLKO.1 216 CDS 100% 4.050 2.025 Y GAGE12I n/a
43 TRCN0000243315 GCCAAATCCAGAGGAGGTGAA pLKO_005 413 CDS 100% 4.050 2.025 Y GAGE12I n/a
44 TRCN0000255386 GTGGAACCAGCAACACCTGAA pLKO_005 222 CDS 100% 4.050 2.025 Y GAGE10 n/a
45 TRCN0000265779 GTGTGAAGATGGTCCTGATGG pLKO_005 377 CDS 100% 4.050 2.025 Y GAGE2A n/a
46 TRCN0000115782 TCCTGAAATGATTGGGCCTAT pLKO.1 176 CDS 100% 4.050 2.025 Y GAGE7 n/a
47 TRCN0000255890 TGAAGCTCATAGCCAGGAACA pLKO_005 332 CDS 100% 4.050 2.025 Y GAGE12E n/a
48 TRCN0000242734 TGATGAAGTGGAACCAGCAAC pLKO_005 215 CDS 100% 4.050 2.025 Y GAGE5 n/a
49 TRCN0000255718 TGGAACCAGCAACACCTGAAG pLKO_005 223 CDS 100% 4.050 2.025 Y GAGE12F n/a
50 TRCN0000432465 TGGGTGTGAGTGTGAAGATGG pLKO_005 368 CDS 100% 4.050 2.025 Y GAGE2C n/a
51 TRCN0000256336 AGGAGATGGACCCGCCAAATC pLKO_005 400 CDS 100% 3.600 1.800 Y GAGE2A n/a
52 TRCN0000243317 CAGGAGATGGACCCGCCAAAT pLKO_005 399 CDS 100% 3.600 1.800 Y GAGE12I n/a
53 TRCN0000243316 GCCGAAGCCTGAAGCTCATAG pLKO_005 323 CDS 100% 3.600 1.800 Y GAGE12I n/a
54 TRCN0000281595 GGAACCAGCAACTCAATGTCA pLKO_005 248 CDS 100% 3.000 1.500 Y GAGE12C n/a
55 TRCN0000262813 ACAGACTGGGTGTGAGTGTGA pLKO_005 362 CDS 100% 2.640 1.320 Y GAGE2B n/a
56 TRCN0000282552 ACCAGCAACTCAATGTCAGGA pLKO_005 251 CDS 100% 2.640 1.320 Y GAGE13 n/a
57 TRCN0000265767 AGCAGTTCAGTGATGAAGTGG pLKO_005 205 CDS 100% 2.640 1.320 Y GAGE12H n/a
58 TRCN0000265850 AGTGGAACCAGCAACACCTGA pLKO_005 221 CDS 100% 2.640 1.320 Y GAGE12E n/a
59 TRCN0000121911 CAGTTCAGTGATGAAGTGGAA pLKO.1 207 CDS 100% 2.640 1.320 Y GAGE7 n/a
60 TRCN0000136873 CAGTTCAGTGATGAAGTGGAA pLKO.1 207 CDS 100% 2.640 1.320 Y GAGE1 n/a
61 TRCN0000415015 CCTGAAGCTCATAGCCAGGAA pLKO_005 330 CDS 100% 2.640 1.320 Y GAGE2C n/a
62 TRCN0000262618 GAAGTGGAACCAGCAACACCT pLKO_005 219 CDS 100% 2.640 1.320 Y GAGE12G n/a
63 TRCN0000155329 GCAGTTCAGTGATGAAGTGGA pLKO.1 206 CDS 100% 2.640 1.320 Y GAGE2C n/a
64 TRCN0000163548 GTGATGAAGTGGAACCAGCAA pLKO.1 214 CDS 100% 2.640 1.320 Y GAGE12I n/a
65 TRCN0000262201 TGAAATGATTGGGCCTATGCG pLKO_005 179 CDS 100% 2.640 1.320 Y GAGE12D n/a
66 TRCN0000265783 TGAAGTGGAACCAGCAACACC pLKO_005 218 CDS 100% 2.640 1.320 Y GAGE2A n/a
67 TRCN0000262812 TGAGTGTGAAGATGGTCCTGA pLKO_005 374 CDS 100% 2.640 1.320 Y GAGE2B n/a
68 TRCN0000243319 AGTGATGAAGTGGAACCAGCA pLKO_005 213 CDS 100% 2.160 1.080 Y GAGE12I n/a
69 TRCN0000371200 ATTATTGGCCTAGACCAAGGC pLKO_005 142 CDS 100% 2.160 1.080 Y GAGE12H n/a
70 TRCN0000282455 CAGTGATGAAGTGGAACCAGC pLKO_005 212 CDS 100% 2.160 1.080 Y GAGE12J n/a
71 TRCN0000263102 CTCAATGTCAGGATCCTGCAG pLKO_005 259 CDS 100% 2.160 1.080 Y GAGE12C n/a
72 TRCN0000138463 CTCCTGAAATGATTGGGCCTA pLKO.1 175 CDS 100% 2.160 1.080 Y GAGE1 n/a
73 TRCN0000262199 GTGAGTGTGAAGATGGTCCTG pLKO_005 373 CDS 100% 2.160 1.080 Y GAGE12D n/a
74 TRCN0000281576 TGTGAAGATGGTCCTGATGGG pLKO_005 378 CDS 100% 2.160 1.080 Y GAGE12C n/a
75 TRCN0000370885 GCCCGAGCAGTTCAGTGATGA pLKO_005 200 CDS 100% 1.650 0.825 Y GAGE6 n/a
76 TRCN0000281542 GCCTAGACCAAGGCGCTATGT pLKO_005 149 CDS 100% 1.650 0.825 Y GAGE12G n/a
77 TRCN0000263103 GGCCGAAGCCTGAAGCTCATA pLKO_005 322 CDS 100% 1.650 0.825 Y GAGE12C n/a
78 TRCN0000243318 GGAGAGGATGAGGGAGCATCT pLKO_005 291 CDS 100% 1.350 0.675 Y GAGE12I n/a
79 TRCN0000369696 TGGCCTAGACCAAGGCGCTAT pLKO_005 147 CDS 100% 1.350 0.675 Y GAGE4 n/a
80 TRCN0000412836 GAGATGGACCCGCCAAATCCA pLKO_005 402 CDS 100% 1.000 0.500 Y GAGE2C n/a
81 TRCN0000412500 AGAGGATGAGGGAGCATCTGC pLKO_005 293 CDS 100% 0.880 0.440 Y GAGE2C n/a
82 TRCN0000371145 AGGAACAGGGTCACCCACAGA pLKO_005 346 CDS 100% 0.880 0.440 Y GAGE2D n/a
83 TRCN0000432757 AGGATGAGGGAGCATCTGCAG pLKO_005 295 CDS 100% 0.720 0.360 Y GAGE7 n/a
84 TRCN0000371198 ATCTGCAGGTCAAGGGCCGAA pLKO_005 308 CDS 100% 0.720 0.360 Y GAGE2D n/a
85 TRCN0000426753 CTCATAGCCAGGAACAGGGTC pLKO_005 337 CDS 100% 0.720 0.360 Y GAGE7 n/a
86 TRCN0000369765 GAAATGATTGGGCCTATGCGG pLKO_005 180 CDS 100% 0.660 0.330 Y GAGE4 n/a
87 TRCN0000370820 CTATGTACAGCCTCCTGAAGT pLKO_005 164 CDS 100% 4.950 2.475 Y GAGE6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001098418.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05736 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_05736 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000467570 TATCCGGGAATCACCCTTACCCAG pLX_317 94.9% 100% 100% V5 n/a
4 ccsbBroadEn_14111 pDONR223 100% 99.4% 94.8% None 148T>C;335delA n/a
5 ccsbBroad304_14111 pLX_304 0% 99.4% 94.8% V5 (not translated due to frame shift) 148T>C;335delA n/a
6 TRCN0000466573 TTGTGTATGCCTCTTGCCACGACC pLX_317 100% 99.4% 94.8% V5 (not translated due to frame shift) 148T>C;335delA n/a
7 ccsbBroadEn_00610 pDONR223 100% 99.1% 97.4% None 67A>G;148T>C;223C>G n/a
8 ccsbBroad304_00610 pLX_304 0% 99.1% 97.4% V5 (not translated due to frame shift) 67A>G;148T>C;223C>G n/a
9 TRCN0000471710 CGGCTTATCACCGACTTTCCCGAT pLX_317 88.1% 99.1% 97.4% V5 (not translated due to prior stop codon) 67A>G;148T>C;223C>G n/a
10 ccsbBroadEn_06249 pDONR223 100% 98.5% 96.5% None (many diffs) n/a
11 ccsbBroad304_06249 pLX_304 0% 98.5% 96.5% V5 (many diffs) n/a
12 TRCN0000478101 CATCGACAATAGGTAGTCAAACGT pLX_317 40.9% 98.5% 96.5% V5 (many diffs) n/a
13 ccsbBroadEn_02972 pDONR223 100% 97.4% 95.7% None (many diffs) n/a
14 ccsbBroad304_02972 pLX_304 0% 97.4% 95.7% V5 (not translated due to frame shift) (many diffs) n/a
15 TRCN0000467585 CGTTGCGCGATTGGCGCAGTTACT pLX_317 88.8% 97.4% 95.7% V5 (many diffs) n/a
16 ccsbBroadEn_00603 pDONR223 100% 81.3% 78.5% None (many diffs) n/a
17 ccsbBroad304_00603 pLX_304 0% 81.3% 78.5% V5 (many diffs) n/a
18 TRCN0000466263 GTCGTATTTAGCCCTAATACTCGG pLX_317 96.3% 81.3% 78.5% V5 (many diffs) n/a
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