Construct: shRNA TRCN0000267622

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_146246.3-3321s21c1
DNA Barcode:
GGACCATGGTGTAAGCTTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rp1l1 (271209)
Gene Description:
retinitis pigmentosa 1 homolog like 1
Transcript:
RefSeq NM_146246.3 (CURRENT)
Match location:
Position 3321 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000267622)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACCATGGTGTAAGCTTATG
Hairpin Sequence:
5'-CCGG-GGACCATGGTGTAAGCTTATG-CTCGAG-CATAAGCTTACACCATGGTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACCATGGTGTAAGCTTATGCTCGAGCATAAGCTTACACCATGGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACCATGGTGTAAGCTTATGCTCGAGCATAAGCTTACACCATGGTCC-3'

Other clones with same target sequence:

(none)