Construct: shRNA TRCN0000267948
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_028372.1-299s21c1
- DNA Barcode:
- TTGCAGGATTGCGGGTCTAAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Mblac2 (72852)
- Gene Description:
- metallo-beta-lactamase domain containing 2
- Transcript:
- RefSeq NM_028372.1 (CURRENT)
- Match location:
- Position 299 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000267948)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TTGCAGGATTGCGGGTCTAAA
- Hairpin Sequence:
-
5'-CCGG-TTGCAGGATTGCGGGTCTAAA-CTCGAG-TTTAGACCCGCAATCCTGCAA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTTGCAGGATTGCGGGTCTAAACTCGAGTTTAGACCCGCAATCCTGCAATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATTGCAGGATTGCGGGTCTAAACTCGAGTTTAGACCCGCAATCCTGCAA-3'
Other clones with same target sequence:
(none)
