Construct: shRNA TRCN0000268031

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001142952.1-482s21c1
DNA Barcode:
TTCTGCCAGAGGGCGTGAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam46c (74645)
Gene Description:
family with sequence similarity 46, member C
Transcript:
RefSeq NM_001142952.1 (CURRENT)
Match location:
Position 482 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268031)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCTGCCAGAGGGCGTGAATA
Hairpin Sequence:
5'-CCGG-TTCTGCCAGAGGGCGTGAATA-CTCGAG-TATTCACGCCCTCTGGCAGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCTGCCAGAGGGCGTGAATACTCGAGTATTCACGCCCTCTGGCAGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCTGCCAGAGGGCGTGAATACTCGAGTATTCACGCCCTCTGGCAGAA-3'

Other clones with same target sequence:

(none)