Construct: shRNA TRCN0000268324

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_029951.1-764s21c1
DNA Barcode:
TCGACAATCAGTTCAAATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
C330007P06Rik (77644)
Gene Description:
RIKEN cDNA C330007P06 gene
Transcript:
RefSeq NM_029951.1 (CURRENT)
Match location:
Position 764 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268324)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGACAATCAGTTCAAATAAT
Hairpin Sequence:
5'-CCGG-TCGACAATCAGTTCAAATAAT-CTCGAG-ATTATTTGAACTGATTGTCGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGACAATCAGTTCAAATAATCTCGAGATTATTTGAACTGATTGTCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGACAATCAGTTCAAATAATCTCGAGATTATTTGAACTGATTGTCGA-3'

Other clones with same target sequence:

(none)