Construct: shRNA TRCN0000268922

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081236.1-2269s21c1
DNA Barcode:
CCAGTGGCATTGGTCATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
2410131K14Rik (76792)
Gene Description:
RIKEN cDNA 2410131K14 gene
Transcript:
RefSeq NM_001081236.1 (CURRENT)
Match location:
Position 2269 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268922)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGGCATTGGTCATATTT
Hairpin Sequence:
5'-CCGG-CCAGTGGCATTGGTCATATTT-CTCGAG-AAATATGACCAATGCCACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGGCATTGGTCATATTTCTCGAGAAATATGACCAATGCCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGGCATTGGTCATATTTCTCGAGAAATATGACCAATGCCACTGG-3'

Other clones with same target sequence:

(none)