Construct: shRNA TRCN0000268998

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001085491.2-202s21c1
DNA Barcode:
CCCATTGAGGCCGGAGACTCC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam229a (68233)
Gene Description:
family with sequence similarity 229, member A
Transcript:
RefSeq NM_001085491.2 (CURRENT)
Match location:
Position 202 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000268998)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATTGAGGCCGGAGACTCC
Hairpin Sequence:
5'-CCGG-CCCATTGAGGCCGGAGACTCC-CTCGAG-GGAGTCTCCGGCCTCAATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATTGAGGCCGGAGACTCCCTCGAGGGAGTCTCCGGCCTCAATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATTGAGGCCGGAGACTCCCTCGAGGGAGTCTCCGGCCTCAATGGG-3'

Other clones with same target sequence:

(none)