Construct: shRNA TRCN0000269320

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001037909.3-1420s21c1
DNA Barcode:
GTGTCAACCCTTTAGATTTAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
C130026I21Rik (620078)
Gene Description:
RIKEN cDNA C130026I21 gene
Transcript:
RefSeq NM_001037909.3 (CURRENT)
Match location:
Position 1420 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000269320)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTCAACCCTTTAGATTTAA
Hairpin Sequence:
5'-CCGG-GTGTCAACCCTTTAGATTTAA-CTCGAG-TTAAATCTAAAGGGTTGACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTCAACCCTTTAGATTTAACTCGAGTTAAATCTAAAGGGTTGACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTCAACCCTTTAGATTTAACTCGAGTTAAATCTAAAGGGTTGACAC-3'

Other clones with same target sequence:

(none)