Construct: shRNA TRCN0000269886

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001098808.1-825s21c1
DNA Barcode:
ATGTCTGCTCACCTAACGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C9orf129 (445577)
Gene Description:
chromosome 9 open reading frame 129
Transcript:
RefSeq NM_001098808.1 (CURRENT)
Match location:
Position 825 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000269886)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTCTGCTCACCTAACGTTT
Hairpin Sequence:
5'-CCGG-ATGTCTGCTCACCTAACGTTT-CTCGAG-AAACGTTAGGTGAGCAGACAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTCTGCTCACCTAACGTTTCTCGAGAAACGTTAGGTGAGCAGACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTCTGCTCACCTAACGTTTCTCGAGAAACGTTAGGTGAGCAGACAT-3'

Other clones with same target sequence:

(none)