Construct: shRNA TRCN0000270076

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001126324.1-1475s21c1
DNA Barcode:
ATATAGAAGGTGAGGTCATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm13083 (279185)
Gene Description:
predicted gene 13083
Transcript:
RefSeq NM_001126324.1 (CURRENT)
Match location:
Position 1475 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000270076)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATATAGAAGGTGAGGTCATTG
Hairpin Sequence:
5'-CCGG-ATATAGAAGGTGAGGTCATTG-CTCGAG-CAATGACCTCACCTTCTATAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATATAGAAGGTGAGGTCATTGCTCGAGCAATGACCTCACCTTCTATATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATATAGAAGGTGAGGTCATTGCTCGAGCAATGACCTCACCTTCTATAT-3'

Other clones with same target sequence:

(none)