Construct: shRNA TRCN0000270078

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001126324.1-1520s21c1
DNA Barcode:
GGTCTCAAGTGGCTCATTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm13083 (279185)
Gene Description:
predicted gene 13083
Transcript:
RefSeq NM_001126324.1 (CURRENT)
Match location:
Position 1520 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000270078)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCTCAAGTGGCTCATTATT
Hairpin Sequence:
5'-CCGG-GGTCTCAAGTGGCTCATTATT-CTCGAG-AATAATGAGCCACTTGAGACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCTCAAGTGGCTCATTATTCTCGAGAATAATGAGCCACTTGAGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCTCAAGTGGCTCATTATTCTCGAGAATAATGAGCCACTTGAGACC-3'

Other clones with same target sequence:

(none)