Construct: shRNA TRCN0000270202

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033782.3-2725s21c1
DNA Barcode:
TGCCAGATCATTGCAACAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm5592 (434172)
Gene Description:
predicted gene 5592
Transcript:
RefSeq NM_001033782.3 (CURRENT)
Match location:
Position 2725 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000270202)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCAGATCATTGCAACAAAG
Hairpin Sequence:
5'-CCGG-TGCCAGATCATTGCAACAAAG-CTCGAG-CTTTGTTGCAATGATCTGGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCAGATCATTGCAACAAAGCTCGAGCTTTGTTGCAATGATCTGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCAGATCATTGCAACAAAGCTCGAGCTTTGTTGCAATGATCTGGCA-3'

Other clones with same target sequence:

(none)